Effect of ipiwi1 RNAi on development of I. pulchra after seven days of ipiwi1 dsRNA treatment. As a control, RNAi with luciferase dsRNA was performed which did not lead to any change in ipiwi1 or ipvasa mRNA expression (A, E), Ipiwi1 protein (C) or cell proliferation (G). After seven days of ipiwi1 RNAi treatment, ipiwi1 and ipvasa mRNA and protein were drastically reduced (B, D, F) and cell proliferation had completely stopped (H). All ipiwi1 knock-down juveniles died before eight days of postembryonic development. In all figures, anterior is to the left. Autofluorescence of diatoms is marked with an asterisk. (t) testes. Scale bars 100 μm.
De Mulder et al. BMC Developmental Biology 2009 9:69 doi:10.1186/1471-213X-9-69