The expression of GMR- lz can rescue cellular defects caused by the overexpression of Ttk69. A. Imaging of Bar positive R1/R6 cells in wt eye discs. Inset in all panels shows higher magnification images. B. Bar expression is lost in GMR-Gal4; UAS-ttk69 discs. C. Partial rescue of R1/6 cells is shown in GMR-Gal4; UAS-ttk69/GMR-lz eye discs. D. Anti-β-galactosidase (β-gal) staining (red) of the Klingon-lacZ line (B38) shows high levels of klingon gene expression in R7 cells and lower expression levels in other cell types. E. B38 enhancer trap activity was only detected at background levels in GMR- Gal4; UAS-ttk69 eye discs. F. Partial rescue of β-gal positive R7 cells is observed in GMR-Gal4; UAS-ttk69/GMR-lz eye discs. D-ECadherin staining (green) in D-F, and G-I was used to mark the furrow. G. Confocal image showing anti-Cut staining (red) of cone cells (CCs) in wt discs. H. Few Cut labelled CCs (red) are detected in GMR-Gal4; UAS-ttk69 discs. I. Partial rescue of CCs is observed in GMR-Gal4; UAS-ttk69/GMR-lz eye discs. J. Imaging of Pros labelled wt eye discs. K. Pros expression is markedly reduced in GMR-Gal4; UAS-ttk69 eye discs. L. A dramatic rescue of Pros in R7 and CCs is observed in GMR-Gal4; UAS-ttk69/GMR-lz eye discs. Scale Bars indicate 20 μm.
Siddall et al. BMC Developmental Biology 2009 9:64 doi:10.1186/1471-213X-9-64