Nucleotide alignment of a conserved region of the lz eye enhancer between D. erecta, D. melanogaster (D.mel) and D. pseudoobscura (D. pseudo). Highly similar sequences to the D. melanogaster lz eye enhancer region were identified in D. erecta and D. pseudoobscura. In this region, four putative Ttk69 core binding sites (red; AGGA-like sequences) were conserved across three species. Binding sites for Sine oculis (green) and Glass (blue), which are known regulators of lz gene expression, are also highly conserved. A conserved ETS binding site (pink) overlapping a Ttk69 core site is shown. A 251 bp minimal enhancer region which is essential for lz expression in undifferentiated cells is depicted (black line). Conserved nucleotides are shaded grey.
Siddall et al. BMC Developmental Biology 2009 9:64 doi:10.1186/1471-213X-9-64