Figure 7.

Tracking of cell movement by monitoring stripe positions during the closure of a central wound. Graphical representation of the healing of a central wound in a PAX6-GFP cornea. A: Time zero showing the extent of the 1 mm wound and the direction of movement of the leading edge of the stripe during wound closure. B: After 18 hours of healing the wound is completely closed but the stripes do not meet in the centre of the original wound. C: Time zero showing the routes taken by tracked stripes. D: After healing it is clear that stripes do not all extend equally, so cells in different places around the wound move at different rates and the wound closes asymmetrically. Scale bar in D = 500 μm.