Figure 7.

mel-46(yt5) mutation causes variable germline defects at 25°C. (A) DIC image of a wild type animal. Arrowheads point to spermatids. (B) DIC image of a mel-46(yt5) homozygous animal. Arrowheads point to sperm cells, arrows point to ooids. (C) mel-46(yt5) homozygous animal of class 1 (see text) stained with DAPI. Arrowheads point to sperm nuclei. Arrows point to ooid nuclei in meiotic prophase, ooid nuclei in diakinesis are circled. TZ: transition zone, the mitotic region is on the left, meiotic pachytene is on the right of TZ. D: mel-46(yt5) homozygous animal of class 2 (see text). The visible portion of the distal arm is seen between the two white strokes. (E) A whole gonad arm from a class 3 mutant stained with DAPI. Detail of two nuclei in diakinesis from a mel-46(yt5) mutant of class 1 (F) and from a mog-3(q74) animal that has produced a few oocytes (G). The six pairs of chromosomes are made visible by DAPI. Open stars designate the proximal end of the germ line, the filled star indicates the distal end. The scale bar represents 10 μm in all panels. Images in panels A to F to were taken with a 40× objective, and those in panels G and H were taken with a 100× objective.