Patterns of cell proliferation in the radial nerve cord. The percentage of BrdU-labeled cells compared to Hoescht labeled nuclei in the (A) ectoneural and (B) hyponeural component of the radial nerve cord are shown. Measurements were done at the injury site and compared with sites proximal and distal to the injury. Animals were injected with BrdU 24 hrs before sacrificed. Quantification of cell division at the injury site shows a peak in cell proliferation at 12 dpi both in the hyponeural and ectoneural components. A smaller increase in cell division is also observed in the nerve stumps proximal to the injury site, but not so distal to the injury. Regenerated tissues in the injured area do not appear until 12-dpe. Each point represents the mean ± S.E. of at least five animals. When compared to distal, significant differences are observed at various stages in the proximal and injured areas *p < .05, **p < .01.
San Miguel-Ruiz et al. BMC Developmental Biology 2009 9:3 doi:10.1186/1471-213X-9-3