Figure 7.

Betacellulin-stimulated plating of islets is blocked when Rac1 function is inhibited. Isolated islets were grown in 5% FCS for two days before switched to 0.5% FCS with and without betacellulin (25 ng/ml) (Day 1). Whereas betacellulin stimulated plating of wild-type islets (on average four days to surpass 15% plating-efficiency compared to six days for unstimulated islets, p < 0.05 in a Student's t-test (n = 6)), plating of RIP-RacN17 islets were unaffected (n = 5). Error bars represent standard error of the mean (s.e.m).