Table 2

genotype

Retina area [μm2 × 106]

RGC layer depth [μm]

Density of Brn3a expressing cells in RGC layer [cells/μm3 × 10-3]

Brn3a expressing cells/retina × 103


dn

vt


Pax6Sey/+

1.74 ± 0.18

17.6 ± 1.4

7.9 ± 0.1

7.4 ± 0.6

241 ± 40

Pax6+/+

2.90 ± 0.24

16.4 ± 1.6

8.9 ± 1.1

8.4 ± 0.3

405 ± 41

PAX77+

1.87 ± 0.24

18.9 ± 3.0

7.5 ± 0.2

7.6 ± 0.2

260 ± 15

PAX77+/+

1.86 ± 0.20

17.9 ± 3.9

8.3 ± 0.3

8.5 ± 0.2

269 ± 28


Numbers and distributions of Brn3a expressing RGCs in Pax6Sey/+, Pax6+/+, PAX77+, and PAX77+/+ embryos. Note that the reduced number of Brn3a-expressing cells in the Pax6Sey/+, PAX77+, and PAX77+/+ embryos compared to Pax6+/+ embryos (see Fig 7 A) appears to correspond to a reduction in retinal area [that is having smaller eyes] as opposed to changes in the density or distribution of Brn3a-expressing cells in the retina. All values mean ± s.e.m. Sample sizes: Pax6Sey/+ n = 5; Pax6+/+ n = 4; PAX77+ n = 3; PAX77+/+ n = 3. Abbreviations: dn, dorsonasal quadrant; vt, ventrotemporal quadrant.

Manuel et al. BMC Developmental Biology 2008 8:59   doi:10.1186/1471-213X-8-59

Open Data