The different retinal cell types are produced normally in the PAX77+/+ eye. Horizontal sections through wild-type and PAX77+/+ eyes. (A) Expression of the RGC marker Brn3a at E18.5. (B) BrdU labelling (brown) of retinal progenitors in S-phase in E18.5 eyes. Sections are counterstained with cresyl violet. Expression of (C, J) Pax6 (green) and GFAP (red), (D) Islet1, (E) Chx10, (F) Syntaxin, (G) R-cadherin, (H) N-cadherin and (I) Brn3a in the retinae of wild-type and PAX77+/+ pups at P6 (C to I) and P14 (J). (C,F) Sections are counterstained with TOPRO3. Arrowheads in C,D,F: amacrine cells; arrows in C,G: horizontal cells; arrows in E: bipolar and progenitor cells; arrowhead in G: outer plexiform layer; arrow in H: outer limiting membrane; stars in C,D,F: ectopic retinal cells; arrowheads in I: RGCs. OL, outer layer; IL, inner layer; ONL, outer nuclear layer; INL inner nuclear layer; RGC, retinal ganglion cells. Scale bars : A, 250 μm; B, 150 μm; E, 300 μm; C,D,F,G,H,I,J, 100 μm.
Manuel et al. BMC Developmental Biology 2008 8:59 doi:10.1186/1471-213X-8-59