Pax6 is overexpressed in the eye of PAX77+/+ embryos. Horizontal sections through the eye of wild-type and PAX77+/+ embryos at (A) E12.5 and (B) E14.5, showing the expression of Pax6/PAX6. (C) Levels of endogenous Pax6 mRNA and total Pax6/PAX6 mRNA in the retina of E14.5 PAX77+/+ embryos, relative to wild-types, determined by real time quantitative RT-PCR and normalised against GAPDH mRNA levels (n = 3 in each case).Pax6 mRNA levels are significantly decreased in the retinae of PAX77+/+ embryos compared to those of wild-types (Student's t-test, p = 0.014) while Pax6/PAX6 mRNA levels are significantly increased (Student's t-test, p < 0.001). Scale bars: A, 200 μm; B, 500 μm.
Manuel et al. BMC Developmental Biology 2008 8:59 doi:10.1186/1471-213X-8-59