Identification of the various loco transcripts in pupae and testes. A: Transcripts loco c1, loco c2, loco c3, and loco c5 were detected in early pupae. B: The transcripts loco c1, loco c2, and loco c3 were detected in late staged pupae, however loco c5 was not. C: Expression of loco c1 was detected in loco318 as was the 5' end of loco c2. However the 3'end of loco c2 (3' c2) was not detected in loco318 as only the non-specific band was produced (Sequencing and alignment showed this band to be CaBP1). Expression of loco c3 was also absent in loco318, however expression of loco c5 remained unaffected. D: Only expression of loco c3 was lost in the pupae of the mutant line loco358. E: All loco transcripts were detected in the pupae of the mutant line loco387. F: Expression of loco c3 was lost in the testes of the mutant line loco358. The true PCR product of loco c2 is marked with an asterisk.
McGurk et al. BMC Developmental Biology 2008 8:37 doi:10.1186/1471-213X-8-37