Table 1

Genotype of colonies isolated after recombineering

Gene

Cassette

PCR product electroporated

Colonies Obtained

GFP + colonies

Cassette + colonies


K10C2.4

galk

FLAG-GFP

714

40/48

3/48

K10C2.4

galk

None

85

0/48

23/48

K10C2.4

RT

FLAG-GFP

55

0/48

46/48

K10C2.4

RT

None

81

0/48

46/48

dod-22

galk

FLAG-GFP

N.D.

46/48

N.D.

dod-22

RT

FLAG-GFP

N.D.

1/48

N.D.

myo-3

galk

FLAG-GFP

N.D.

46/47

N.D.

myo-3

RT

FLAG-GFP

N.D.

2/48

N.D.

dod-22

(Frozen 1 year)

galk

FLAG-GFP

N.D.

16/48

N.D.

myo-3

(Frozen 1 year)

galk

FLAG-GFP

N.D.

18/48

N.D.


Zhang et al. BMC Developmental Biology 2008 8:119   doi:10.1186/1471-213X-8-119

Open Data