Table 1 |
|||||
|
Genotype of colonies isolated after recombineering |
|||||
|
Gene |
Cassette |
PCR product electroporated |
Colonies Obtained |
GFP + colonies |
Cassette + colonies |
|
|
|||||
|
K10C2.4 |
galk |
FLAG-GFP |
714 |
40/48 |
3/48 |
|
K10C2.4 |
galk |
None |
85 |
0/48 |
23/48 |
|
K10C2.4 |
RT |
FLAG-GFP |
55 |
0/48 |
46/48 |
|
K10C2.4 |
RT |
None |
81 |
0/48 |
46/48 |
|
dod-22 |
galk |
FLAG-GFP |
N.D. |
46/48 |
N.D. |
|
dod-22 |
RT |
FLAG-GFP |
N.D. |
1/48 |
N.D. |
|
myo-3 |
galk |
FLAG-GFP |
N.D. |
46/47 |
N.D. |
|
myo-3 |
RT |
FLAG-GFP |
N.D. |
2/48 |
N.D. |
|
dod-22 (Frozen 1 year) |
galk |
FLAG-GFP |
N.D. |
16/48 |
N.D. |
|
myo-3 (Frozen 1 year) |
galk |
FLAG-GFP |
N.D. |
18/48 |
N.D. |
|
|
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|
Zhang et al. BMC Developmental Biology 2008 8:119 doi:10.1186/1471-213X-8-119 |
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