Generation of transgenic animals with modified fosmids. The modified fosmid carrying the K10C2.4:FLAG-GFP transgene was introduced into the DP38 (unc-119(ed3)) strain via microparticle bombardment. (A) GFP expression seen in the transgenic worms. (B and C) GFP expression is reduced by treatment with GFP (B) or K10C2.4 (C) RNAi. (D) Detection of the FLAG-GFP transgene in RNAi treated worm extracts by western blotting with anti-FLAG antibodies. Equal loading was confirmed by blotting with anti-actin antibodies.
Zhang et al. BMC Developmental Biology 2008 8:119 doi:10.1186/1471-213X-8-119