Production of FLAG-GFP modified fosmids with pMOD4-galK-G. The fosmid carrying the K10C2.4 gene was modified using positive and negative selection using PCR amplified cassettes from the indicated plasmids. The effectiveness of fosmid modification was monitored via PCR using the indicated oligo sets. Lane 1, 2, 3, 4, 5, 6, 8 and 9 FLAG-GFP F and FLAG-GFP R; Lane 7 RT screen F and RT screen R.
Zhang et al. BMC Developmental Biology 2008 8:119 doi:10.1186/1471-213X-8-119