Additional file 2.

lef1 is necessary for expression from foxP2-enhancerA.1, foxP2-enhancerB, and foxP2-enhancerD. Whole-mount confocal images at 52 hpf (except foxP2-enhancerD), lateral view, anterior left, dorsal up. Conditions (wild type- wt, or lef1 morphant- lef1 MO) are shown above the panels, while the enhancer name is to the left of the panels. (Abbreviations: hb: hindbrain; tec: tectum; tel: telencephalon.) (A) Tg(foxP2-enhancerA.1:EGFP)zc44 expresses in telencephalon, tectum, and hindbrain. (B) Tg(foxP2-enhancerA.1:EGFP)zc44 embryo injected with lef1 morpholino: GFP expression is absent in the tectum and hindbrain (arrows), although telencephalic expression persists. (C) Tg(foxP2-enhancerB:EGFP)zc41 expresses in telencephalon, tectum, and hindbrain. (D) Tg(foxP2-enhancerB:EGFP)zc41 embryo injected with lef1 morpholino: GFP expression is absent in the hindbrain (arrow), but still present in the telencephalon. (E) Tg(foxP2-enhancerD:EGFP)zc47 expresses in tectum. (F) Tg(foxP2-enhancerD:EGFP)zc47 embryo injected with lef1 morpholino: GFP expression is absent in the tectum (arrow). (G) Tg(elavl3:EGFP)zf8 embryo shows GFP expression in all post-mitotic neurons. (H) Tg(elavl3:EGFP)zf8 embryo injected with lef1 morpholino: GFP expression is still present in the tectum and hindbrain. (I) Tg(pax2a:GFP)e1 embryo shows expression in the MHB and hindbrain. (J) Tg(pax2a:GFP)e1 embryo injected with lef1 morpholino has persistent expression in the MHB and hindbrain.

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Bonkowsky et al. BMC Developmental Biology 2008 8:103   doi:10.1186/1471-213X-8-103