Additional file 1.

Loss of lef1 leads to absent foxP2 expression in the tectum, mid-hindbrain boundary, and hindbrain. Whole-mount in situs at 48 hpf; anterior to left, dorsal up, eyes removed. Scale bar = 50 μm. (Conditions: hs:Δtcf- Tg(hsp70l:Δtcf:GFP)w26, lef1 mo- lef1 morphants, wt- wild type, x8- homozygous Df(LG01)x8. Abbreviations: hb: hindbrain; mhb: mid-hindbrain boundary; tec: tectum; tel: telencephalon.) (A, B) lef1 morphant (B) lacks expression in tectum and has diminished expression in the hindbrain (arrows), compared to wild-type (A). (C) In Df(LG01)x8 homozygote, expression is absent in the tectum and hindbrain (arrows). (D) In Tg(hsp70l:Δtcf:GFP)w26 embryo, collected four hours after heat shocking, expression is absent from the tectum and hindbrain (arrows). MHB expression is reduced but not absent, perhaps because lef1 induction of foxP2 expression is already sufficiently established by the time of heat-shock.

Format: TIFF Size: 6.3MB Download file

Bonkowsky et al. BMC Developmental Biology 2008 8:103   doi:10.1186/1471-213X-8-103