Figure 5.

A: Hematopoietic progeny obtained from OrgD3 electroporated YS. GFP⁺ cells display hematopoietic markers indicating that pre-hematopoietic mesodermal cells were targeted. B: GFP⁺ cells sorted from electroporated OrgD3-YS give rise, after a 5 day culture on OP9 stroma, to the hematopoietic progeny typical of the YS, i.e. few precursors (left panel), myeloid (middle panel) and erythroid (right panel) cells. C: Frequency of hematopoietic precursors within OrgD3 control YS (see also Table 1) obtained by limiting dilution assay.