Defects in wing development upon PKD overexpression. A) Wild type wing with five longitudinal (L1 – L5) and anterior and posterior cross veins (acv, pcv). The posterior compartment is highlighted in yellow. B) Ubiquitous PKD-WT overexpression affects the posterior cross vein (arrow). C) A similar defect is observed upon overexpression of PKD-WT just in the posterior compartment (arrow). D) Overexpression of PKD-kd caused loss of veins (arrow) and induced ectopic veinlets (arrowhead). E) In contrast, overexpression of PKD-kd within the posterior compartment only resulted primarily in loss of veins (arrow) and only few ectopic veinlets (arrowhead). F) Overexpression of PKD-SE within the posterior compartment prevented cross vein formation (arrow). G) Depletion of PKD within the posterior wing compartment by RNAi caused loss of wing tissue, resulting in a conspicuous bent of the wing when compared to wild type. It did not affect wing venation; however, the texture of the wing was altered. A dotted line marks the antero-posterior compartment border. H) Tissue loss was not influenced by concomitant overexpression of DIAP1, albeit the total size of the wing was increased (compare with G). I) In contrast, a complete rescue was observed by concurrent overexpression of PKD-WT. Genotypes are: A) da-Gal4/+, UAS-GFP/+; B) da-Gal4/UAS-PKD-WT10-1; C) en-Gal4/UAS-PKD-WT10-1; D) da-Gal4/UAS-PKD-kd102-1; E) en-Gal4/UAS-PKD-kd102-1; F) en-Gal4/+, UAS-PKD-SE112-6 /+; G) en-Gal4/+, UAS-dsPKD124-4 /+; H) UAS-DIAP1/+, en-Gal4/+, UAS-dsPKD124-4 /+; I) en-Gal4/UAS-PKD-WT10-1, UAS-dsPKD124-4 /+. Scale bar, 200 μm.
Maier et al. BMC Developmental Biology 2007 7:74 doi:10.1186/1471-213X-7-74