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Open Access Highly Accessed Research article

Protein Kinase D regulates several aspects of development in Drosophila melanogaster

Dieter Maier1, Anja C Nagel1, Helena Gloc13, Angelika Hausser2, Sabrina J Kugler1, Irmgard Wech1 and Anette Preiss1*

Author Affiliations

1 Universität Hohenheim, Institut für Genetik (240), Garbenstr. 30, 70599 Stuttgart, Germany

2 Universität Stuttgart, Institut für Zellbiologie und Immunologie, Allmandring 31, 70569 Stuttgart, Germany

3 present address: Universitätsklinikum Würzburg, Medizinische Klinik II, Josef-Schneider-Str. 2, 97080 Würzburg, Germany

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BMC Developmental Biology 2007, 7:74  doi:10.1186/1471-213X-7-74

Published: 25 June 2007

Abstract

Background

Protein Kinase D (PKD) is an effector of diacylglycerol-regulated signaling pathways. Three isoforms are known in mammals that have been linked to diverse cellular functions including regulation of cell proliferation, differentiation, motility and secretory transport from the trans-Golgi network to the plasma membrane. In Drosophila, there is a single PKD orthologue, whose broad expression implicates a more general role in development.

Results

We have employed tissue specific overexpression of various PKD variants as well as tissue specific RNAi, in order to investigate the function of the PKD gene in Drosophila. Apart from a wild type (WT), a kinase dead (kd) and constitutively active (SE) Drosophila PKD variant, we also analyzed two human isoforms hPKD2 and hPKD3 for their capacity to substitute PKD activity in the fly. Overexpression of either WT or kd-PKD variants affected primarily wing vein development. However, overexpression of SE-PKD and PKD RNAi was deleterious. We observed tissue loss, wing defects and degeneration of the retina. The latter phenotype conforms to a role of PKD in the regulation of cytoskeletal dynamics. Strongest phenotypes were larval to pupal lethality. RNAi induced phenotypes could be rescued by a concurrent overexpression of Drosophila wild type PKD or either human isoform hPKD2 and hPKD3.

Conclusion

Our data confirm the hypothesis that Drosophila PKD is a multifunctional kinase involved in diverse processes such as regulation of the cytoskeleton, cell proliferation and death as well as differentiation of various fly tissues.