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Open Access Highly Accessed Research article

Generation of FGF reporter transgenic zebrafish and their utility in chemical screens

Gabriela A Molina1, Simon C Watkins2 and Michael Tsang13*

Author Affiliations

1 Department of Molecular Genetics and Biochemistry, University of Pittsburgh, School of Medicine. BST3-5062, 3051 Fifth Avenue, Pittsburgh, PA 15213, USA

2 Department of Cell Biology and Physiology, Center for Biological Imaging, University of Pittsburgh, School of Medicine S362 Biomedical Science Towers, 3500 Terrace Street. Pittsburgh, PA 15261 USA

3 LMG/NICHD/NIH, Building 6B, 9000 Rockville Pike. Bethesda, MD 20892 USA

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BMC Developmental Biology 2007, 7:62  doi:10.1186/1471-213X-7-62

Published: 6 June 2007

Additional files

Additional file 1:

Movie showing d2EGFP expression in Dorsal Forerunner Cells (DFCs). Movie of developing Tg(Dusp6:d2EGFP)pt6 embryo from late gastrula stage until bud stage. The movie shows a group of fluorescent cells, the DFCs as they coalesce towards the caudal region of the embryo.

Format: MOV Size: 416KB Download file

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Open Data

Additional file 2:

Movie showing d2EGFP expression in the developing embryo. Time-laspe imaging of developing Tg(Dusp6:d2EGFP)pt6 embryo from gastrula stage until 24-somite stage. Movie highlights the dynamic expression of d2EGFP within the developing embryo. The movie of the fluorescent embryo was overlayed onto the DIC-imaged movie to show clearly the developing embryo. Note expression of d2EGFP within the developing hindbrain, MHB and Kupffer's vesicle.

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Additional file 3:

Movie showing d2EGFP expression in Kupffer's vesicle. Time-laspe imaging of Tg(Dusp6:d2EGFP)pt6 embryo from gastrula stage until 24-somite stage. GFP movie showing the formation of Kupffer's vesicle from the 6-somite stage right until after this structure collapses at the 20-somite stage. Note Kupffer's vesicle cells collapse inwards and begin to migrate towards the tail bud.

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Additional file 4:

Movie showing d2EGFP expression in Kupffer's vesicle with DIC overlay. This is the same movie as Supplemental Movie 3, but overlayed with the DIC-imaged movie to show structures of the developing embryo.

Format: MOV Size: 9.9MB Download file

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Additional file 5:

Diagram of the small molecules used in this study. The majority of the chemicals used in the pilot screen are related in structure and contain the indolinone backbone as described for SU5402.

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