Figure 5.

Loss of pRb stimulates Indian hedgehog expression. (A) RT-PCR from P0 intestines derived from RB^+/+, RB^+/- and RB^-/- newborn pups were performed to detect the expression levels of Shh, Ihh, Ptch1, Ptch2, Gli1, Gli2, Gli3 and actin used as a control. (B) Immunofluoresence analysis of Ihh in intestine sections derived from RB^+/+, RB^+/- and RB^-/- newborn pups was performed by using anti-Ihh antibody. Arrows indicate regions of increased anti-Ihh immunostaining.