Additional file 2.

Silencing of OCT4 and NANOG in 2102Ep, NTERA2, and two batches of NCCIT cells. (A) Real-time PCRs at two timepoints after esiRNA transfection using OCT4, NANOG, and SOX2 primers as well as markers of trophoblast differentiation. Bars indicate standard errors between two independent experiments (NCCIT) or between values based on distinct housekeeping controls (2102Ep and NTERA2). (B) Representative cellular morphology at day 4. See Fig. 3C for undifferentiated samples. (C) Real-time PCR-based comparison between cells kept undifferentiated by high density passaging vs. cells plated at low density as required for efficient gene silencing, using CDX2 as an early differentiation marker.

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Greber et al. BMC Developmental Biology 2007 7:46   doi:10.1186/1471-213X-7-46