Figure 4.

Progenitor cell fate is not changed in the mutant retinas of the Rybp heterozygous eyes. Wild type (A, C, E) and Rybp heterozygous null eyes exhibiting retinal coloboma (B, D, F) were stained with TUJ1 (A, B), NeuN (C, D) and Nestin (E, F) at E14.5. (A-B) TUJ1 staining, marking early neuronal cell types including ganglion cells of the retina, is comparable in the wildtype and mutant mice. (C-D) Similarly, NeuN, a postmitotic neural marker, shows no significant alteration in the mutant retinas. (E-F) The distribution of nestin, an intermediate filament marker for neural progenitor cells, is not affected in the mutant retinas. L; lens, NR; neuroretina, Magnifications: (×460)

Pirity et al. BMC Developmental Biology 2007 7:39   doi:10.1186/1471-213X-7-39
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