Regulation of NOGO-A by FGF in the early chick embryo. A) Schematic of an early primitive streak stage chick embryo (HH4) illustrating the location of bead placement (anterior-right) within the area pellucida between the neural plate and the area opaca (outer gray). B) FGF4 upregulation of a pre-neural marker, SOX3, 3 hours after application. C, F) PBS-soaked beads failed to induce the pre-neural marker or NOGO-A. D, E) NOGO-A induction following FGF4 application was upregulated after 3 hours and expression was maintained around the bead following 9 hours of incubation. Scale bar 250 μm. "*" initial position of bead in E) prior to processing.
Caltharp et al. BMC Developmental Biology 2007 7:32 doi:10.1186/1471-213X-7-32