Table 4

Fertilization defects associated with mfr mutations

Male Genotype*

# of Eggs Examined

Percent Inseminated Eggs

Sperm Head Condensed

Subsequent Development(number of eggs)


mfrZ0695/Df

40

86%

100%

mfrZ6248/Df

31

85%

100%

mfrZ2942/Df

30

100%

93%

Sperm decondensation (1); Pronuclear apposition (1)

mfrZ3281/Df

30

92%

92%

Sperm decondensation (1); Pronuclear apposition (1)

mfrZ2713/Df

29

100%

90%

Pronuclear apposition (3)


* males express dj-GFP as sperm tail marker; Df is Df(3L)h-i22

percentage of inseminated eggs relative to a control cross of dj-GFP males mated to wild-type females; DJ-GFP was detected in 87% of the eggs

sperm chromatin visualized by DAPI staining

Smith and Wakimoto BMC Developmental Biology 2007 7:21   doi:10.1186/1471-213X-7-21

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