Deglycosylation of neonatal and adult sRAGE and mRAGE. Neonatal (0–24 hrs) and adult soluble and membrane rat lung preparations were treated with N-glycosidase F and compared to untreated controls. After deglycosylation neonatal sRAGE bands resolved to match that of the adult sRAGE and neonatal mRAGE matched that of adult mRAGE (n = 2). The two bands seen for both sRAGE and mRAGE reveals a partial and complete deglycosylation.
Lizotte et al. BMC Developmental Biology 2007 7:15 doi:10.1186/1471-213X-7-15