Efficient Otx2-GFP detection during late-development and in adults. a-j. Direct visualisation of GFP fluorescence on 10μm thin section of E16.5 (a-d), P2 (e-h) and adult (i-j) Otx2+/OG animals. b-d. Magnifications of part delimitated by the corresponding red boxes in image a. f-h. Magnifications of part delimitated by the corresponding red boxes in image e. Insets in a and e are Hoechst staining of the same sections (left) or control fluorescence of 10 μm thin sections of Otx2+/+ animals (right). Anterior is leftward. k-m. Comparison of Otx2-GFP detection by anti-GFP antibody (k) or direct GFP fluorescence (l) in the ventral lateral geniculate nucleus of adult Otx2+/OG animals. cb, cerebellum; cp, choroid plexus; cx, cortex; di, diencephalon; ic, inferior colliculus; mes, mesencephalon; oe, olfactory epithelium; sc, superior colliculus. Scale bars: 500 μm (a, e, i, j) and 10 μm (k-m).
Fossat et al. BMC Developmental Biology 2007 7:122 doi:10.1186/1471-213X-7-122