FACS of hESC for array analysis. Gates are set relative to isotype controls. A, hESC were separated by FACS according to staining intensity for GCTM-2 and TG30 (CD9) into 4 populations; P4 (GCTM-2-CD9-), P5 (GCTM-2LOWCD9LOW), P6 (GCTM-2MIDCD9MID) and P7 (GCTM-2HIGHCD9HIGH). B, heat map depicting normalized intensity of gene expression for genes from P7 V P4 experiment with a B stat greater than zero.
Laslett et al. BMC Developmental Biology 2007 7:12 doi:10.1186/1471-213X-7-12