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Open Access Research article

Implantation Serine Proteinases heterodimerize and are critical in hatching and implantation

Navneet Sharma, Shiying Liu, Lin Tang, Jackie Irwin, Guoliang Meng and Derrick E Rancourt*

Author Affiliations

Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Calgary, Heritage Medical Research Building, 3330 Hospital Dr. NW, Calgary AB, T2N 4N1, Canada

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BMC Developmental Biology 2006, 6:61  doi:10.1186/1471-213X-6-61

Published: 11 December 2006

Additional files

Additional File 1:

Purification of ISP1-ISP2 enzyme complex. A – Coomassie stained 8% native polyacrylamide gel showing a single homogenous band obtained after purification of enzyme complex as described in the text. B – Calibration plot of a standard mix of proteins run on Superdex-75 showing the native molecular weight of the ISP enzyme complex.

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Additional File 2:

Inhibitors & ISP. Table 1 – Effect of some general serine protease inhibitors on ISP enzyme activity. Table 2 – In vitro hatching data. Table 3 – In vitro outgrowth data.

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