|
Distribution of genes with expression levels <50 and >50–10,000 as detected by Illumina bead array in 8 hESC populations All human ESC samples were hybridized in one experiment and the relative detection levels of genes were binned to obtain a global overview of transcription, approximately 8, 000 genes (~50%) were greater than 100 arbitrary units. The numbers are similar to results obtained by other large scale analysis such as MPSS. |
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| Abundance (relative detection levels) |
H9 |
H9 on human feeders |
I6 |
BG01 |
BG02 |
BG03 |
BG01V |
Pooled (H1, H7, H9) |
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|
|
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| No. |
% |
No. |
% |
No. |
% |
No. |
% |
No. |
% |
No. |
% |
No. |
% |
No. |
% |
|
|
|
||||||||||||||||
| <50 |
2,909 |
21.2 |
6,067 |
38.4 |
3,559 |
25.3 |
4,528 |
30.7 |
4,256 |
30.5 |
4,706 |
32.2 |
419 |
4.3 |
5,803 |
34.4 |
| >50 |
10,817 |
78.8 |
9,747 |
61.6 |
10,484 |
74.7 |
10,232 |
69.3 |
9,694 |
69.5 |
9,915 |
67.8 |
9,438 |
95.7 |
11,085 |
65.6 |
| >100 |
8,126 |
59.2 |
7,539 |
47.7 |
7,409 |
52.8 |
7,703 |
52.2 |
7,496 |
53.7 |
7,430 |
50.8 |
7,230 |
73.3 |
8,217 |
48.7 |
| >500 |
3,077 |
22.4 |
2,947 |
18.6 |
2,626 |
18.7 |
3,065 |
20.8 |
2,950 |
21.1 |
2,852 |
19.5 |
2,851 |
28.9 |
2,941 |
17.4 |
| >1000 |
1,629 |
11.9 |
1,625 |
10.3 |
1,490 |
10.6 |
1,638 |
11.1 |
1,589 |
11.4 |
1,517 |
10.4 |
1,566 |
15.9 |
1,554 |
9.2 |
| >5000 |
248 |
1.8 |
257 |
1.6 |
256 |
1.8 |
256 |
1.7 |
263 |
1.9 |
262 |
1.8 |
275 |
2.8 |
251 |
1.5 |
| >10000 |
90 |
0.7 |
94 |
0.6 |
112 |
0.8 |
92 |
0.6 |
100 |
0.7 |
107 |
0.7 |
101 |
1.0 |
94 |
0.6 |
| Total No. of genes detected at >0.99 confidence |
13,726 |
15,814 |
14,043 |
14,760 |
13,950 |
14,621 |
9,857 |
16,888 |
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Liu et al. BMC Developmental Biology 2006 6:20 doi:10.1186/1471-213X-6-20 |
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