Figure 2.

Shaggy (sggM1-1) and Disheveled (dsh477), components of the wingless pathway, are involved in cell cycle control, but Pygopus (pygo) is not. (A) Quantification of the number of cells in Sgg mutant clones compared to the number in their sister clones shows that they are twice as large. (B) Quantification of the number of cells in Dsh mutant clones compared to the number in their sister clones shows that they are half the size of their twin spots (p = 0.0005). (C) However, quantification of the number of cells in Pygo mutant clones compared to the number in their sister clones shows that there is no affect on cell cycle when Pygo expression is lost. (D, D') Sgg clones (dashed line) show prolonged expression of CycB (red) and PH3 (blue) indicating that mitoses are still occurring in this stage 9 egg chamber. After the mitotic-to-endocycle transition, Sgg mutant clones show upregulation of Notch (E, E') and stg-lac Z (F, F'). (G) Premature expression of pUASpDelta-1 and pUASpDelta-2 causes a 60% and 89% reduction in the number of PH3 positive cells respectively (n = 37 matTub-Gal4; pUASpDl-1; n = 10 pUASpDl-2/matTub-Gal4; n = 30 control). (H) In control ovarioles, PH3 (green) is expressed at stage 6. (I) Driving Delta (red) prematurely in the germline causes all or some of the follicle cells to cease division early (as visualized by an absence of PH3 positive cells). Clones are marked with loss of GFP and indicated by dashed lines.

Jordan et al. BMC Developmental Biology 2006 6:16   doi:10.1186/1471-213X-6-16
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