Figure 6.

Confocal images of sea urchin embryos at various developmental stages. The cellular distribution of MVP is revealed by staining with an affinity-purified polyclonal antiserum against the sea urchin MVP and secondary staining with Oregon Green-conjugated anti-rabbit antibody (A, D, G, J; left column). The nuclei are stained with DAPI (B, E, H, K; middle column). By merging the MVP and DNA images coincident staining is observed as cyan (C, F, I, L; right column). Note that as development proceeds the cellular localization of MVP progressively shifts from cytoplasmic to nuclear.