Additional File 1.

Figure 1. Method for RNAi in peri-implantation development. Schematic representation of the proposed method for transient loss of gene function by electroporation followed by uterine transfer and development in utero. Approaches for the assessment of gene-specific knock-down and phenotypic analyses are suggested. Table 1. Proportion of dsBmp4 RNA-electroporated embryos with defective expression of indicated marker genes. Group 1, embryos morphologically normal (MN) and developmentally delayed or arrested (DA); group 2, morphologically defective embryos (Df). SExp, symmetrically expanded expression around the distal tip; DR, distally (DVE) restricted expression; Abs, absence of expression.

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Soares et al. BMC Developmental Biology 2005 5:28   doi:10.1186/1471-213X-5-28