Table 3

Identification of Wnt-1 and β-catenin responsive genes in a twelve hour co-culture assay and a stable cell line overexpressing the non-degradable β-catenin mutant 4145β-catenin. Differential screening was performed using labeled cDNA isolated from a twelve hour Wnt-1 co-culture and a stably transfected C57MG cell line that overexpresses the stable β-catenin mutant 4145β-catenin. The labeled cDNA was hybridized overnight with membranes spotted with the putative Wnt-1 responsive sequences. Signals were quantified using the PhosphorImager, and the genes were classified based on their response to Wnt-1 signaling and high levels of β-catenin.

    Genes Induced by Both Wnt-1 and b-catenin


Elf-1

HP1BP38

tlm

cul-2

activin

FIN-16

Tob5

Calponin

Calmodulin

IGFBP5

EI24

Thrombospondin

Proteasome component C8

Fibronectin receptor

GDP dissociation inhibitor

rab11b

Wnt-5A

WISP-1

WISP-2

WISP-3

Thymosin b4

Connexin43


Taneyhill and Pennica BMC Developmental Biology 2004 4:6   doi:10.1186/1471-213X-4-6

Open Data