Table 2

Summary of co-culture time course data. C57MG cells were co-cultured with QT6 or QT6Wnt-1 cells for the indicated times. Total RNA was isolated from each co-culture, and Northern blot analysis was performed using probes to the selected genes. Signals were quantified using a PhosphorImager. The reported value (fold induction) is the measure of the ratio of the signal for each gene in the Wnt-1 co-culture (normalized to GAPDH) to the signal for each gene in the control co-culture (normalized to GAPDH), averaged from three independent experiments. Zero (0) indicates that no detectable difference in gene expression was observed between the Wnt-1 and control co-cultures. "inc." signifies an increase in gene expression in the Wnt-1 co-culture, with no detectable signal in the control co-culture. Negative values indicate a decrease in expression.

Time (hours)

Time (days)

Gene

2

3

6

12

24

48

3

7

Connexin43

1.5

1.4

1.9

2.3

Protein phosphatase V

2

1.2

1.6

1

SPARC

1.5

1.2

1.3

2.9

-2

-1.5

Radixin

1.5

1.5

1.3

1.6

Elf-1

1.5

1.3

1.9

1.4

Thymosin b4

1.2

0

0

1.1

WISP-1

0

1.1

2

1.5

WISP-2

1.2

1.7

3

-1.4

Collagen XII

inc.

inc.

2.2

inc.

-4

HP1BP38

3.9

1.3

-4.6

-1.2

Myc

1.9

1.8

-3.6

0

Thrombospondin

1.5

0

2.3

1.7

1.4

1

-7

1.3

Cyclin G

inc.

0

1.4

1.4

1.3

1.2

-1.5

-1.6

GKLF

1.6

1.4

1.2

1.1

1.4

1.5

BTEB2

3.6

5.7

8.3

8

11

35

4.5

2.1

Taneyhill and Pennica BMC Developmental Biology 2004 4:6   doi:10.1186/1471-213X-4-6