Semi-quantitative RT-PCR (QRT-PCR) confirmation of putative Wnt-1 responsive genes after retroviral infection of C57MG cells. C57MG cells were infected with either a Wnt-1 or empty vector retrovirus. Forty-eight hours post-infection, cells were split into media containing 2.5 μg/ml puromycin. Infected cells were passaged in the presence of puromycin for three weeks after which time total RNA was isolated from each cell line. Reverse transcription was performed on the indicated amounts of total RNA, followed by PCR using primers designed for each sequence of interest and TaqMan probes (Applied Biosystems). triose phosphate isomerase (tpi) was used as a control in each QRT-PCR experiment.
Taneyhill and Pennica BMC Developmental Biology 2004 4:6 doi:10.1186/1471-213X-4-6