Table 2

Primers used in quantitative RT-PCR.

HPRT:

forward primer

5'-TGGAAAGAATGTCTTGATTGTTGAA-3'

reverse primer

5'-AGCTTGCAACCTTAACCATTTTG-3'

BMP-4:

forward primer

5'-GCTTGAGTACCCGGAGCGT-3'

reverse primer

5'-TGTTCTTCGTGATGGAAACTCCT-3'

Plasminogen activator inhibitor-1:

forward primer

5'-GGCCTCCAAAGACCGGA-3'

reverse primer

5'-CAGCATAGCCAGCACCGA-3'

Osteoblast specific factor 2:

forward primer

5'-AAGGAAAAGGGTCATACACGTACTTC-3'

reverse primer

5'-TGCGAATGTCAGAATCCAGGT-3'

Flt4 ligand:

forward primer

5'-CAGCTGCGGAAAGGCG-3'

reverse primer

5'-TTTACACTGTCCCCTGTCCTGG-3'

HTK ligand:

forward primer

5'-GCAGGAGACACCGCAAACA-3'

reverse primer

5'-GTGGCCAGTGTGCTGAGAGA-3'

Mesothelin:

forward primer

5'-GCAGTCAGGGAGGTTCTGAGG-3'

reverse primer

5'-GGTGGAGACTGACCACTTCGA-3'

Stromelysin-1:

forward primer

5'-TGGCCATCTCTTCCATCCAA-3'

reverse primer

5'-CCCAGAACTGATTTCCTTTAAAAATG-3'

Groucho related protein 1

forward primer

5'-CCCTATGCCCCTCATTGGA-3'

reverse primer

5'-CCGTGGTTGAGGGTGTTGAT-3'

Retinoic acid receptor-alpha:

forward primer

5'-AGCACCAGCTTCCAGTCAGTG-3'

reverse primer

5'-CTGCTGCTCTGGGTCTCGA-3'

Id4:

forward primer

5'-GAACAAGCAGGGTGACAGCA-3'

reverse primer

5'-CCGCACACCTGGCCAT-3'

Neural-restrictive silencer factor:

forward primer

5'-CGGAAGACAAATGCAGGAGTTC-3'

reverse primer

5'-TTCGGCTTCGTACTGGCAA-3'

Bcl3:

forward primer

5'-GCACGGCGCCAACGT-3'

reverse primer

5'-GCCAGACGCAGAATGCAGA-3'

Krox-20:

forward primer

5'-GCGGGAGATGGCATGATC-3'

reverse primer

5'-GAAGCTACTCGGATACGGGAGA-3'

PTX3:

forward primer

5'-TGGACAACGAAATAGACAATGGA-3'

reverse primer

5'-CTGGCGGCAGTCGCAT-3'

Bradykinin B1 receptor:

forward primer

5'-TCAACTGGCCCTTTGGAAGT-3'

reverse primer

5'-AACAGGTTGGCCTTGATGACC-3'

Guanine nucleotide-binding protein:

forward primer

5'-CTGGTAGAACAGCTGAAGATGGAAG-3'

reverse primer

5'-ACAGTAGGCCATCAAGTCAGCA-3'

ATTP signaling molecule:

forward primer

5'-TGCTCCACCCATTTGCTTCT-3'

reverse primer

5'-TGGCATCCACATGTTTTCCA-3'

Son of sevenless 1:

forward primer

5'-CCTGAAAGCCCTCCCTTGTT-3'

reverse primer

5'-AGATGTAATGGTGAGCTTGAGAAAAC-3'

SHPS-1:

forward primer

5'-GGTCAACATCTTCCACACGGTT-3'

reverse primer

5'-TTTGTGTCCTGGATCTGGGTTA-3'

TRAF1:

forward primer

5'-TGTGTGGCCGGACTGTCA-3'

reverse primer

5'-AGCGCAGGCACAACTTGTAAC-3'

IL-2 receptor gamma:

forward primer

5'-TTGGTGCAGTACCGGAGCA-3'

reverse primer

5'-GCAGGGAGAATCTAGGTTCATGA-3'

Transferrin receptor:

forward primer

5'-GACGCTTTGGGTGCTGGT-3'

reverse primer

5'-GGCAAGTTTCAACAGAAGACCTGT-3'

IL-6:

forward primer

5'-TTACACATGTTCTCTGGGAAATCG-3'

reverse primer

5'-AAGTGCATCATCGTTGTTCATACA-3'

Ki-67 antigen:

forward primer

5'-TTTCAGGTCTCTGGAAGCAGTCA-3'

reverse primer

5'-ATCTCCATAATTGCTTTGATTGCA-3'

Semaphorin F:

forward primer

5'-AGTGGTGAGCTCTATGCTGCAA-3'

reverse primer

5'-CCCAGACTTCGGTAAATGGC-3'

DM-GRASP:

forward primer

5'-GCAAAACTGATTGTGGGAATTG-3'

reverse primer

5'-CAGTAGACGACACCAGCAACG-3'


Shown is a list of forward and reverse primers specific to the murine genes used in quantitative RT-PCR.

Prieve and Moon BMC Developmental Biology 2003 3:2   doi:10.1186/1471-213X-3-2

Open Data