Table 1

Genes differentially regulated by Wnt-5a/Rfz-2 and Wnt-1 in C57mg cells.

nd, not determined

Avg. fold changes

Functional Category of Genes

GenBank acc. no.

Wnt-5a Rfz-2

Wnt-1

Microarray

Quant. RT-PCR

Microarray

Quant. RT-PCR


Cell Growth/Differentiation

BMP-4

L47480

-3

-2.4

1.4

1.2

Cell Adhesion

plasminogen activator inhibitor-1

M33960

-2.8

-2.4

2.1

1.2

osteoblast specific factor 2

D13664

-2.4

-1.6

1.4

1.1

Flt4 ligand

U58112

3.3

2

1

1.2

fibronection

ET62894

2

nd

-1.8

nd

HTK ligand

L38847

-2.1

-1.9

2.5

3.2

mesothelin

D86370

-4

-2.9

8.4

2.8

stromelysin 1

X66402

3.8

4.4

-1.6

-2.5

Transcriptional Regulator

groucho-related gene 1 protein

U61362

-2.1

-1.6

1.4

-1.4

retinoic acid receptor-alpha

X57528

-2.3

-2

1

-1.3

Id4 helix-loop-helix protein

X75018

-4.1

-3.6

1.6

-1.1

neural-restrictive silencer factor

U13878

-3.5

1.1

1.6

1.8

bcl-3

M90397

1.9

2.2

-2

-1.4

Krox-20

X06746

-3.4

-1.7

6.2

1.5

Inflammation

PTX3

X83601

-2.5

-2.1

1.6

1.6

bradykinin B1 subtype receptor

U47281

-2.5

4

2.7

3.2

Signaling

Rho-associated protein kinase

U58513

-1.2

nd

-4.9

nd

guanine nucleotide-binding protein

W83658

1.6

1.7

-4.4

-4.4

signaling molecule (ATTP)

U97571

-1.3

1.5

-3.5

-2.7

son of sevenless 1

Z11574

2.5

2.9

-2.6

-2

SHPS-1

D87967

-2.7

-1.4

3

-1.7

TRAF1

L35302

2.6

1.5

2

1.7

Cell surface receptor

interleukin 2 receptor gamma

U21795

2

2.7

-1.4

1.9

transferrin receptor

X57349

-1.6

-1.6

-2.3

-1.9

Cytokine

interleukin-6

X54542

2.1

-1.1

1.6

1

Cell Cycle

Ki-67 antigen

ET62993

-1.45

-1.1

-6.5

-5.8

Axon Guidance

semaphorin F

X97817

2.5

3.1

-2

-4

DM-GRASP

L25274

3.3

1.8

-2.8

-2.6


Acc., accession; Avg., average; Quant., quantitative; BMP-4, bone morphogenetic protein-4; HTK ligand, hepatoma transmembrane kinase ligand; PTX3, pentraxin 3; SHPS-1, src homology protein substrate-1; TRAF1, TNF receptor associated factor-1. Shown is a list of genes that are differentially regulated by Wnt-5a/Rfz2 and Wnt-1 in C57mg cells identified by DNA microarray analysis. The values determined by DNA microarray analysis were validated using quantitative RT-PCR. Average fold changes from DNA microarray analysis and quantitative RT-PCR were determined from two independent experiments and from three independent experiments performed in triplicate respectively.

Prieve and Moon BMC Developmental Biology 2003 3:2   doi:10.1186/1471-213X-3-2

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