Induction of mesothelin and SL-1 expression by Wnt-1 or Wnt-5a signaling. A. RT-PCR was performed on RNA obtained from 3 separate experiments in which C57mg cells were co-cultured with 293T cells expressing Wnt-1, Wnt-5a, or CS2+ as control. The amounts of mesothelin and SL-1 mRNAs were normalized to HPRT and plotted as fold increase relative to C57mg cells co-culture with 293T cells expressing CS2+. B. C57mg cells (wt) or C57mg cells stably expressing the Rfz-2/β2AR chimeric receptor were treated with the β2AR agonist isoproterenol or the antagonist propranolol as control for 8 hours prior to harvesting for RNA extraction and RT-PCR analysis. The amounts of mesothelin and SL-1 mRNAs were normalized to HPRT and plotted as fold increase relative to propranolol treated cells. Mean levels were determined from 3 separate experiments.
Prieve and Moon BMC Developmental Biology 2003 3:2 doi:10.1186/1471-213X-3-2