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Resolution: standard / high Figure 3.
Sagittal section of stage 21 chick embryo immunostained with an anti-trypsin antibody.
The olfactory nerve (ON, arrow) was immunoreactive for trypsin as were regions of
the developing telencephalon (TEL). As a control, typsin protein was incubated with
the anti-trypsin antibody for pre-absorption. This antigen/antibody complex was then
used to immunostain sagittal sections of stage 21 chicks. Panel B demonstrates that
pre-absorption eliminated all immunoreactivity in the head demonstrating the specificity
of trypsin immunolocalization in experimental sections.
Drapkin et al. BMC Developmental Biology 2002 2:1 doi:10.1186/1471-213X-2-1 |
