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Open Access Research article

Metabolomic insights into system-wide coordination of vertebrate metamorphosis

Taka-Aki Ichu123, Jun Han2, Christoph H Borchers12, Mary Lesperance3 and Caren C Helbing1*

Author Affiliations

1 Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada

2 University of Victoria – Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, Victoria V8Z 7X8, BC, Canada

3 Department of Mathematics and Statistics, University of Victoria, Victoria, BC V8W 3R4, Canada

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BMC Developmental Biology 2014, 14:5  doi:10.1186/1471-213X-14-5

Published: 5 February 2014

Abstract

Background

After completion of embryogenesis, many organisms experience an additional obligatory developmental transition to attain a substantially different juvenile or adult form. During anuran metamorphosis, the aquatic tadpole undergoes drastic morphological changes and remodelling of tissues and organs to become a froglet. Thyroid hormones are required to initiate the process, but the mechanism whereby the many requisite changes are coordinated between organs and tissues is poorly understood. Metabolites are often highly conserved biomolecules between species and are the closest reflection of phenotype. Due to the extensive distribution of blood throughout the organism, examination of the metabolites contained therein provides a system-wide overview of the coordinated changes experienced during metamorphosis. We performed an untargeted metabolomic analysis on serum samples from naturally-metamorphosing Rana catesbeiana from tadpoles to froglets using ultraperformance liquid chromatography coupled to a mass spectrometer. Total and aqueous metabolite extracts were obtained from each serum sample to select for nonpolar and polar metabolites, respectively, and selected metabolites were validated by running authentic compounds.

Results

The majority of the detected metabolites (74%) showed statistically significant abundance changes (padj < 0.001) between metamorphic stages. We observed extensive remodelling of five core metabolic pathways: arginine and purine/pyrimidine, cysteine/methionine, sphingolipid, and eicosanoid metabolism and the urea cycle, and found evidence for a major role for lipids during this postembryonic process. Metabolites traditionally linked to human disease states were found to have biological linkages to the system-wide changes occuring during the events leading up to overt morphological change.

Conclusions

To our knowledge, this is the first wide-scale metabolomic study of vertebrate metamorphosis identifying fundamental pathways involved in the coordination of this important developmental process and paves the way for metabolomic studies on other metamorphic systems including fish and insects.

Keywords:
Postembryonic development; Thyroid hormone; Metamorphosis; Metabolites; Serum; Ultra-performance liquid chromatography; Quadrupole time-of-flight; Mass spectrometry; Vertebrate