Additional file 2: Figure S2.

Specificity of the anti-DGKθ antibody against DGKθ and other DGKs. EGFP-fused DGKθ [45] and DGKβ [13] were transiently expressed in HeLa cells and cultured for 24 hr. The cells were fixed with 4% paraformaldehyde in PBS (-) and permeabilized with 0.3% Triton X -100 in PBS (-). Immunofluorescence staining was performed using the indicated antibodies followed by usual method [46]. Scale bar, 20 μm.

Format: PDF Size: 192KB Download file

This file can be viewed with: Adobe Acrobat Reader

Ueda et al. BMC Developmental Biology 2013 13:35   doi:10.1186/1471-213X-13-35