Additional file 2: Figure S2.
Specificity of the anti-DGKθ antibody against DGKθ and other DGKs. EGFP-fused DGKθ  and DGKβ  were transiently expressed in HeLa cells and cultured for 24 hr. The cells were fixed with 4% paraformaldehyde in PBS (-) and permeabilized with 0.3% Triton X -100 in PBS (-). Immunofluorescence staining was performed using the indicated antibodies followed by usual method . Scale bar, 20 μm.
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Ueda et al. BMC Developmental Biology 2013 13:35 doi:10.1186/1471-213X-13-35