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Open Access Research article

Expression pattern of Protein Kinase C ϵ during mouse embryogenesis

Sergio Carracedo*, Ursula Braun and Michael Leitges

Author Affiliations

Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, Oslo, N-0349, Norway

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BMC Developmental Biology 2013, 13:16  doi:10.1186/1471-213X-13-16

Published: 2 May 2013

Abstract

Background

Protein kinase C epsilon (PKCϵ) belongs to the novel PKC subfamily, which consists of diacylglycerol dependent- and calcium independent-PKCs. Previous studies have shown that PKCϵ is important in different contexts, such as wound healing or cancer. In this study, we contribute to expand the knowledge on PKCϵ by reporting its expression pattern during murine midgestation using the LacZ reporter gene and immunostaining procedures.

Results

Sites showing highest PKCϵ expression were heart at ealier stages, and ganglia in older embryos. Other stained domains included somites, bone, stomach, kidney, and blood vessels.

Conclusions

The seemingly strong expression of PKCϵ in heart and ganglia shown in this study suggests a important role of this isoform in the vascular and nervous systems during mouse development. However, functional redundancy with other PKCs during midgestation within these domains and others reported here possibly exists since PKCϵ deficient mice do not display obvious embryonic developmental defects.

Keywords:
Novel protein kinase C; PKC epsilon; Mouse embryogenesis; Lac Z; Ganglia