Additional file 8.

Figure S7: Caffeine shown early gene differentiation in cells pulsed with cAMP. A) AX2 cells were incubated at a density of 1 × 107 cells/ml in the Sorensen buffer containing either adenosine or caffeine and were pulsed with 30 nM cAMP for 5 hours for every six minutes intervals. The cAMP treated cells were developed on non nutrient agar plate in the presence or absence of either adenosine or caffeine. B) We checked the expression of acaA and cAR1 genes of pulsed AX2 cells in the presence or absence of either caffeine or adenosine by performing qRT-PCR. The values represent mean ± standard deviation from three independent experiments. C) Cell adhesion protein (Cad-1 and CsaA) and the early gene induction marker protein (CsaA) expressions: To check expression levels, we performed Western blot of these proteins in AX2 cells pulsed with 30 nM cAMP in the presence or absence of adenosine or caffeine.

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Jaiswal et al. BMC Developmental Biology 2012 12:5   doi:10.1186/1471-213X-12-5