The aggregate formed in the presence of ammonia were comparatively larger than the respective controls. Ammonia was generated by mixing 2 ml of 2 mM NH4Cl solution and 2 ml of 1N NaOH solution . A total of 4 ml solution was poured on the top lid and the dishes having the cells (plated on agar media) were kept inverted. To prevent the diffusion of gas, the plates were tightly sealed with the parafilm. Scale bar = 1000 μm.
Jaiswal et al. BMC Developmental Biology 2012 12:5 doi:10.1186/1471-213X-12-5