Additional file 3 Figure S3.

Lack of autophagy does not affect EGFR signaling activity. HS-FLP induced ATG1 clones (marked by the lack of GFP) were examined for different read-outs of EGFR signaling. (A, B) Gurken (Grk) protein (stained in red) is translated by the oocyte and activates the EGF receptor in adjacent FCs. Normal accumulation of Grk in the posterior corner of the oocyte (arrowhead) and uptake of Grk by FCs (arrows) are seen in stage 7 egg chambers (A-A”), as well as after the movement of the nucleus to the anterior-dorsal side in stage 10 (B-B”). (C, D) The transcription factor Broad is expressed in all stage 9 oocyte-associated FCs and no difference is seen between WT and ATG1 mutant FCs (outlined in yellow) (C-C”). By stage 10, Broad gets repressed in midline FCs and all other FCs except the two patches of future roof cells, which is equally seen in WT and ATG1 mutant FCs (outlined in yellow) (D-D”). (E, F) In eggs containing ATG1 mutant FCs, a normal distribution of kekkon (kek, stained in red) expression is seen in FCs overlying the nucleus in stage 10 eggs (E-E”) and also in stage 11 eggs in cells that later form the dorsal appendages (F-F”). Anterior is to the left, posterior to the right. In B and E, dorsal is to the top. Scale bar: 50 μm. Genotypes: A-D: ATG1∆ 3D-FRT80B/FRT80-UbiGFP. E, F: P[w+ lac-Z]BB142 (=kekkon-lacZ); ATG1∆ 3D-FRT80B/FRT80-UbiGFP.

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Barth et al. BMC Developmental Biology 2012 12:35   doi:10.1186/1471-213X-12-35