Additional file 1 Figure S1.
Generation of ATG mutant FC clones by different techniques. (A-B’) Producing germline clones (GLCs) using the HS-FLP FRT OvoD technique induces a complete mutant germline since GCs homozygous for the dominant female sterile mutation OvoD die. However, mutant clones are also induced in the somatic tissue where the mutation is not lethal. Thus, eggs with a mosaic FC epithelium occur and develop (A-B’, arrowheads, marked by the lack of GFP). (C-D’) For comparison, HS-FLP induced FC clones (ATG1 mutant clones are marked with GFP) (C, C’) and e22c-GAL4, UAS>FLP induced ATG1 FC clones (mutant clones are marked by the lack of GFP) are shown (D, D’). Anterior is to the left, posterior to the right. Scale bar: 50 μm. Genotypes: A: hs flp/+; OvoDFRT80B/FRT80B-UbiGFP, B: hs flp/+; OvoDFRT80B/ATG1∆ 3DFRT80B-UbiGFP, C: hs flp/+; w+FRT80B/ATG1∆ 3DFRT80B-UbiGFP, D: hs flp/+; e22c UAS>FLP; FRT80B-UbiGFP/ATG1∆ 3DFRT80B-UbiGFP.
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Barth et al. BMC Developmental Biology 2012 12:35 doi:10.1186/1471-213X-12-35