Lack of ATG1 in FCs affects proper egg development. Heat shock flipase (HS-FLP) mediated generation of FC clones mutant for ATG1 (marked by the lack of GFP) caused a wide array of oogenesis defects. Compound eggs with more than 16 cysts (outlined in yellow) (A, A’) and ovarioles lacking stalk cells (E, E’) were often observed. Some eggs consisted of less than the 16 germline cyst normally present in WT eggs (outlined in yellow) (B, B’). Inverted eggs presenting the oocyte in the wrong position (outlined in yellow) (C, C’) or eggs with two oocytes (outlined in yellow) (D, D’) were less frequently observed. Mature eggs regularly lack DAs (arrowheads) (F, G). Stage 14 egg chambers often contain persisting nurse cell nuclei (PNCN) (arrowheads) (I, I’) that are normally degenerated in control eggs with WT clones (H, H’). Anterior is to the left, posterior to the right, except F, G: anterior to the top, posterior to the bottom, dorsal to the front. Scale bar: 50 μm Genotypes: A-E, G and I: hs flp/+; ATG1∆ 3DFRT80B/FRT80B-UbiGFP. F and H: hs flp/+; FRT80Biso/FRT80B-UbiGFP .
Barth et al. BMC Developmental Biology 2012 12:35 doi:10.1186/1471-213X-12-35