Research article
Regulation of multiple tip formation by caffeine in cellular slime molds
Department of Biotechnology, Indian Institute of Technology-Madras, Chennai 600036, India
BMC Developmental Biology 2012, 12:26 doi:10.1186/1471-213X-12-26
Published: 28 August 2012Additional files
Movie 1A. Cell movement in control slug.
Format: AVI Size: 7.9MB Download file
Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more
Movie 1B. Cell movement in slug in the presence of caffeine (during multiple tip formation).
Format: AVI Size: 5.3MB Download file
Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more
Additional file 3:
Figure S1. Effect of caffeine analogs (5 mM) on secondary tip formation. Theophylline, paraxanthine, theobromine and adenosine were checked for the secondary tip formation in slugs D. discoideum. Slugs in the presence of these compounds culminated except caffeine which induced secondary tip formation. Scale bar = 200 μm.
Format: PDF Size: 93KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 4:
Figure S2. Effect on caffeine and adenosine on TipA and TipB mutants at mound stage. Both compounds showed no response in promoting multiple tips. Scale bar = 200 μm. (PDF 101 kb)
Format: PDF Size: 101KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 5:
Figure S3. Effect of 1 mM caffeine on fruiting bodies of P. pallidum. Scale bar=1000 μm.
Format: PDF Size: 51KB Download file
This file can be viewed with: Adobe Acrobat Reader
