The response to knockdown of MBNL1 is temporally regulated. AVC explants from stage 14, 15, or 16 were transfected with or without MBNL1 siRNA and the number of invaded cells was counted at 38 hrs. The mean number of invaded cells per explant + the standard error of the mean is shown. Levels of invasion in stage 14 AVC explants treated with MBNL1 siRNA are comparable to control stage 15 explants, and levels in stage 15 AVC explants treated with MBNL1 siRNA are comparable to control stage 16 explants. Knockdown of MBNL1 did not stimulate invasion in stage 16 explants. An asterisk indicates a significant difference from control explants from the same stage (P ≤ 0.05).
LeMasters et al. BMC Developmental Biology 2012 12:22 doi:10.1186/1471-213X-12-22